Step by step user guide on how to use OkaGel liquid from the box to usable application
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- Choose the appropriate model required. Note that LAP, Irgacure, and Eosin Y require different wavelengths of light to be activated.
- Calculate the volume of GelMA and the volume of the photoinitiator needed to achieve the desired concentrations.
- Transfer the photoinitiator to the OkaGel liquid with a sterile pipette or syringe. Handle in an area with appropriate exhaust ventilation.
- Mix well for 15 minutes under heat (37°C) shielded from light.
- Use immediately or store at 4°C protected from light for future use.
- If stored at 4°C, warm the OkaGel solution to 37°C to liquefy and vortex to mix prior to use.
- Use warm water to clean up Okagel residue.
– Wear laboratory personnel protective equipment (eye protection, lab coat and gloves) when handling Okagel.
– Store away from light and moisture, keep the container tightly closed.
– Avoid temperatures above 60 ° C and below freezing.
Loessner, D., Meinert, C., Kaemmerer, E., Martine, L., Yue, K., Levett, P., . . . Hutmacher, D. . Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nature Protocols, 11(4), 727-746.(2016). Retrieved October 14, 2022 from 10.1038/nprot.2016.037.
Gelma casting protocol – CELLINK. CellLink. (2018, October 3). Retrieved October 14, 2022, from https://www.cellink.com/wpcontent/uploads/2018/04/GelMACastingProtocolver1.pdf Section 1. Identification – Gelma Co. The GelMA Company. (n.d.). Retrieved October 14, 2022, from https://okasciences.com/wp-content/uploads/2022/04/GELMA20Liquid20SDS.pdf
Viability report: Lap and Irgacure with gelma. Allevi. (2020, March 14). Retrieved October 14, 2022, from https://www.allevi3d.com/lap-and-irgacure-viability-with-gelma/