LIGHT BOX Protocol
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Overview:
The light box is a simple wooden container containing a light source on the top. The light source in the light box will have a wavelength of either 405nm or 520nm. Okagel samples are placed inside the box, and will photo-crosslink under the light source.
Protocol:
- The light source at the top of the box has an adjustable screw lens. Turn the lens to adjust the diameter of light (light spot area).
- Place the Okagel sample in the centre of the light box. Close the door. For small samples ( Ex. 1 drop),crosslinking will take 1-2 minutes. For large samples (Ex. full well plate), crosslinking will take a maximum of 4-5 minutes.
Notes
Stiffness of the sample will change based on the intensity of light used, and the duration of time the sample is left inside the container.
